CRISPR's Next Advance Is Bigger Than You Think | Jennifer Doudna | TED

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[Research Frontier] New Trends in Prime Editing: Point Mutated Cells for Preclinical Gene Therapy

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  Prime editing is an improved gene editing technology based on the CRISPR/Cas system to achieve precise fragment insertion, deletion, and arbitrary substitution of bases at the target site of the genome, with higher accuracy and lower error rate. When it came out, it attracted widespread attention from many scientific researchers, and extensive research was conducted on the treatment of hereditary diseases, gene mutation diseases, and cancers. For example, correcting the mutant genes to treat genetic diseases or editing oncogenes in cancer cell genomes to prevent them from growing and spreading. The following three articles are about the clinical treatment of Prime Editing, bringing you the latest research trends.   Prime editing helps in the treatment of β - thalassemia, and no off-target  effect was detected         β - thalassemia is a hematopoietic system disease caused by a single gene mutation in the hemoglobin (HGB) subunit beta gene (HBB), res...
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Prime editing 101：what is prime editing? | EDITGENE

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Prime editing is an   advanced   gene editing technology proposed by David R. Liu's team in 2019. It can mediate targeted insertions, deletions, and twelve base substitutions without DSBs and donor DNA templates to achieve precise gene editing. Prime editing has developed rapidly in the past 4   years and has shown great potential in various fields.   Prime Editing  mechanism The prime editing system consists of Prime editing guide RNA (pegRNA) and nCas9. PegRNA can be divided into a guide RNA at the 5' end, a Cas9 binding region in the middle, and a PBS (Prime-binding site) at the 3' end; nCas9 is a Cas9 nickase with single-stranded cleavage activity and fused with M-MLV reverse transcriptase. The complex recognizes PAM under the g uidance of pegRNA   and binds to the target DNA strand complementary to the guide RNA. Cas9 enzyme cuts the 3rd and 4th nucleotides of the original spacer sequence upstream of PAM to generate a nick; the broken DNA single strand...
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