CRISPR Geneome Editing Blogger - CRISPR Gene Editing New - EDITGENE

Overview of the Lipoprotein Response Pathway What is the Lipoprotein Response? The Response to Lipoprotein Particle Pathway refers to the key biological processes by which cells or organisms react to stimulation by lipoprotein particles such as LDL, HDL, and VLDL (Gene Ontology term: GO:0055094). Lipoproteins are responsible for transporting lipids, including cholesterol, triglycerides, and phospholipids, through the bloodstream. Maintaining their metabolic homeostasis is essential for cellular function, energy balance, and endocrine regulation. Disruption of lipoprotein metabolism can directly lead to major metabolic diseases, including atherosclerosis, hyperlipidemia, non-alcoholic fatty liver disease (NAFLD), obesity, and type 2 diabetes. Regulatory Network of the Lipoprotein Response The Response to Lipoprotein Particle Pathway regulates multiple biological processes, including lipid uptake, inflammatory responses, cholesterol homeostasis, and innate immune signaling. When lipoprot...

KRAS is one of the most frequently mutated oncogenes in human cancers, yet different mutation subtypes (G12C, G12D, G12V, G13D, etc.) exhibit significant differences in oncogenic potential, signaling pathway preferences, and drug sensitivity. The approval of the first G12C inhibitor in 2021 ushered in a new era of precision targeting of KRAS. Understanding allele-specific functions, screening sensitive drugs, and dissecting resistance mechanisms all rely on isogenic cell models with consistent genetic backgrounds. This article systematically reviews the clinical significance and therapeutic breakthroughs of major KRAS mutation subtypes, and introduces EDITGENE’s off-the-shelf cell lines with KRAS point mutation in HCT116 and LLC, built on the Bingo™ PE7 platform .  These ready-to-ship cell lines provide academic institutes and pharmaceutical companies with ideal tools ranging from in vitro screening to in vivo efficacy evaluation. KRAS mutation spectrum: heterogeneous distribution...

  Mitochondrial autophagy, also known as mitophagy, is a selective form of autophagy that specifically removes damaged or superfluous mitochondria from cells. It plays a central role in maintaining mitochondrial quality control, reducing the accumulation of reactive oxygen species (ROS), and regulating cellular energy metabolism and homeostasis. In recent years, a growing body of research has demonstrated that dysregulation of mitophagy is closely associated with a wide range of diseases, including neurodegenerative disorders, cancer, cardiovascular diseases, and metabolic dysfunction. Therefore, systematically elucidating its molecular mechanisms and validating its functions using precise models has become a major focus of current research. Mechanisms of Mitophagy At its core, mitophagy involves the sequestration of specific mitochondria by autophagosomes, followed by their delivery to lysosomes for degradation. This process typically includes several key steps: ● Damage recogniti...