CRISPR Geneome Editing Blogger - CRISPR Gene Editing New - EDITGENE

  When the metabolic balance of lipoprotein particles in the blood is disrupted, it triggers chronic inflammation—the common root of atherosclerosis, metabolic syndrome, and even neurodegenerative diseases. Circulating lipoprotein particles are the primary carriers of cholesterol and triglycerides: low-density lipoprotein (LDL) delivers cholesterol to tissues, while high-density lipoprotein (HDL) recycles it via reverse transport. Together, they maintain the delicate balance of lipid metabolism. When this balance is broken—such as through oxidative deposition of LDL or dysfunction of HDL—damage-associated molecular patterns (DAMPs) like oxidized phospholipids (OxPLs) activate innate immune signaling, driving foam cell formation and metabolic inflammation. Pathway enrichment analysis shows that lipoprotein response pathways are significantly enriched in functional genomics (FDR = 1.11×10⁻⁵), further confirming their role as key regulatory hubs. With CRISPR gene editing technology, i...

  Mitochondrial autophagy, also known as mitophagy, is a selective form of autophagy that specifically removes damaged or superfluous mitochondria from cells. It plays a central role in maintaining mitochondrial quality control, reducing the accumulation of reactive oxygen species (ROS), and regulating cellular energy metabolism and homeostasis. In recent years, a growing body of research has demonstrated that dysregulation of mitophagy is closely associated with a wide range of diseases, including neurodegenerative disorders, cancer, cardiovascular diseases, and metabolic dysfunction. Therefore, systematically elucidating its molecular mechanisms and validating its functions using precise models has become a major focus of current research. Mechanisms of Mitophagy At its core, mitophagy involves the sequestration of specific mitochondria by autophagosomes, followed by their delivery to lysosomes for degradation. This process typically includes several key steps: ● Damage recogniti...

  CRISPR library screening   is a powerful high-throughput method for gene analysis, leveraging the CRISPR/Cas9 system. This approach involves constructing a library containing thousands of sgRNAs, which are inserted into lentiviral vectors and used to infect target cells at a low multiplicity of infection (MOI). This ensures that each cell receives only one sgRNA, enabling accurate functional gene screening. In this article, we highlight the top three most popular libraries of the month and share key research papers to provide guidance, insights, and support for advancing your scientific studies. I. Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins Original Link: https://doi.org/10.1016/j.celrep.2023.112065 Four-transmembrane proteins belong to a superfamily of membrane proteins. They were first identified while searching for new cell surface antigens in mammalian cancer cells. Currently, 33 members of this four-trans...