CRISPR Geneome Editing Blogger - CRISPR Gene Editing New - EDITGENE

CRISPR detection technology is a gene editing technology based on the CRISPR-Cas system for precise editing and detection in the genome. The development of CRISPR detection technology can be divided into the following stages: Initial discovery stage: In the 1980s, scientists discovered the CRISPR-Cas system. This discovery laid the foundation for subsequent gene editing technology. Technology development stage: In the early 21st century, with the development of genomics and molecular biology, scientists began to study how to use the CRISPR-Cas system for gene editing. After a series of research and exploration, the CRISPR-Cas9 system was finally developed, a powerful gene editing tool that can achieve precise editing and detection in the genome. Application stage: As the CRISPR-Cas9 system continues to be improved and optimized, its application scope is becoming more and more extensive. At present, CRISPR detection technology has been widely used in basic research, biotechnology, medi...

Prime editing is an   advanced   gene editing technology proposed by David R. Liu's team in 2019. It can mediate targeted insertions, deletions, and twelve base substitutions without DSBs and donor DNA templates to achieve precise gene editing. Prime editing has developed rapidly in the past 4   years and has shown great potential in various fields.   Prime Editing  mechanism The prime editing system consists of Prime editing guide RNA (pegRNA) and nCas9. PegRNA can be divided into a guide RNA at the 5' end, a Cas9 binding region in the middle, and a PBS (Prime-binding site) at the 3' end; nCas9 is a Cas9 nickase with single-stranded cleavage activity and fused with M-MLV reverse transcriptase. The complex recognizes PAM under the g uidance of pegRNA   and binds to the target DNA strand complementary to the guide RNA. Cas9 enzyme cuts the 3rd and 4th nucleotides of the original spacer sequence upstream of PAM to generate a nick; the broken DNA single strand...

  The  nucleic acid   testing for quick  specific ity  detection of infectious   pathogens is  widely  recognized, such as  P olymerase  C hain  R eaction (PCR) . However,  it is expensive   due to the  expensive equipment   and  the  highly trained Researchers . In this way,  CRISPR-Cas   systems have recently emerged as an alternative to PCR-based diagnosis. CRISPR diagnosis is also known as next-generation molecular diagnostic technology, which mainly utilizes RNA to detect biomarkers in biological fluids ( such as blood or urine ). By processing the  sample  preparation  (including pre-amplification), target recognition, signal release,   and  testing, t he CRISPR/Cas system   becomes the  potential diagnostic tool for disease and pathogen detection . It is  reported  that  CRISPR/Cas biosensing systems have the advantages of high resolutio...