CRISPR Geneome Editing Blogger - CRISPR Gene Editing New - EDITGENE

 1.  K nockout cells decipher the mechanism of  cell  antiviral ability   Guanylate-binding prot eins (GBPs) are highly expressed interferon-stimulated genes (ISGs) that have antagonistic effects on various intracellular pathogenic microorganisms. ECTV has very similar  genetic and disease characteristics  to the monkeypox virus, making it a valuable research tool for studying orthopoxvirus-host interactions. Guanylate-binding proteins (GBPs) are highly expressed interferon-stimulated genes (ISGs) that have antagonistic effects on various intracellular pathogenic microorganisms. The gRNA plasmid containing the specific  targeting gene PAPL   was transfected into small hamster kidney cells (BRT17 cells), and then the small hamster kidney cells were infected by ECTV, and subsequently obtained the PAPL knockout ECTV. Viral plaque assays were performed in African green monkey kidney fibroblasts (CV1) to evaluate the effect of PAPL  deletion...

CRISPR technology provides a powerful tool for genetic manipulation and functional genomics research. CRISPR libraries refer to a set of gRNA libraries that are designed and constructed to screen and identify genes that affect a particular phenotype or disease process. CRISPR libraries contain a large number of guide RNA sequences, each of which is specifically designed to target a different gene . By transfecting the gRNA library into cells and screening for desired phenotypes, researchers can identify candidate genes that may be involved in the regulation of the phenotype or disease process. CRISPR libraries have been widely used in basic research, drug discovery, and disease treatment. Compared with traditional cDNA and RNAi libraries, CRISPR libraries have a number of advantages, including higher targeting efficiency, lower noise, and more targeted knockout . Here are some suggestions on how to effectively utilize CRISPR libraries to speed up your research: 1. Target gene selection...

The British drug regulatory agency MHRA (Medicines and Healthcare Products Regulatory Agency) announced on its official website that it has approved the  CRISPR gene editing therapy Casgevy (exa-cel) . British regulators stated that this is the world's first gene editing therapy approved to treat this indication, and also the world's first approved therapy using the "gene scissors" CRISPR. This also marks that this breakthrough technology has become a practical treatment only 11 years after it first appeared in 2012. It is worth mentioning that Emmanuelle Charpentier, who jointly won the Nobel Prize in Chemistry for this breakthrough technology in 2020, is one of the co-founders of CRISPR Therapeutics. UK Greenlight

With the rapid development of CRISPR/Cas9 gene editing technology, CRISPR library screening has played an important role in drug screening, viral infection, and tumor functional gene screening experiments. Compared with cDNA libraries and RNAi libraries, CRISPR library screening has the advantages of versatility, low noise, high knockout efficiency, and low off-target rate, making it a popular method for candidate gene screening. Q: What is CRISPR library screening? A: CRISPR library screening is a method for accurate screening for functional genes. Q: What ’ s the difference between two plasmid library and one plasmid library？ One plasmid system: It includes a promoter, gRNA, Cas9, and selection marker. gRNA and Cas9 can be simultaneously transferred into cells. However, due to the large size of the one plasmid system, its infection efficiency is low. Two plasmid system: only carries gRNA and the promoter; Cas9 stable cell line gen...

  Applications of CRISPR Library Screening In the past few years, CRISPR technology has become a new gem, and CRISPR library screening is one of its applications. By using the whole genome knockout libraries or sub-pool knockout libraries, sgRNAs of genes related to certain phenotypes will be enriched. This high-throughput functional screening technique has gradually replaced RNAi and cDNA libraries, providing a high-throughput and efficient screening tool for the study of functional genes. Common CRISPR sgRNA library screening applications are as follows: ·  Exploring the mechanism of drug action, identifying and validating drug targets. ·    Discovering cancer treatment targets by analyzing upstream and downstream regulatory mechanisms. ·   Exploring the therapeutic mechanisms of metabolic diseases by analyzing metabolic pathway regulation mechanisms. The following 3 cases are typical applications of CRISPR library screening. Case 1: Screening for new therapeu...