How to utilize CRISPR library to speed up your research?
With the rapid development of CRISPR/Cas9 gene editing technology, CRISPR library screening has played an important role in drug screening, viral infection, and tumor functional gene screening experiments. Compared with cDNA libraries and RNAi libraries, CRISPR library screening has the advantages of versatility, low noise, high knockout efficiency, and low off-target rate, making it a popular method for candidate gene screening. Q: What is CRISPR library screening ? A: CRISPR library screening is a method for accurate screening for functional genes. Q: What ’ s the difference between two plasmid library and one plasmid library？ A: One plasmid system: It includes a promoter, gRNA, Cas9, and selection marker. gRNA and Cas9 can be simultaneously transferred into cells. However, due to the large size of the one plasmid system, its infection efficiency is low. Two plasmid system: only carries gRNA...