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Human Whole Genome CRISPR/Cas9 Knockout Library and Human RNA Binding Protein Library

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  CRISPR library screening   is a powerful high-throughput method for gene analysis, leveraging the CRISPR/Cas9 system. This approach involves constructing a library containing thousands of sgRNAs, which are inserted into lentiviral vectors and used to infect target cells at a low multiplicity of infection (MOI). This ensures that each cell receives only one sgRNA, enabling accurate functional gene screening. In this article, we highlight the top three most popular libraries of the month and share key research papers to provide guidance, insights, and support for advancing your scientific studies. I. Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins Original Link: https://doi.org/10.1016/j.celrep.2023.112065 Four-transmembrane proteins belong to a superfamily of membrane proteins. They were first identified while searching for new cell surface antigens in mammalian cancer cells. Currently, 33 members of this four-trans...

CRISPR gene editing combined with magnetocaloric activation: A new strategy to assist precision medicine in cancer

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In current cancer treatment, inducing tumor cell to apoptosis is a key strategy, since tumor cells gain uncontrolled proliferation ability by mechanism of inhibiting normal apoptosis. Gene editing technology, especially the CRISPR-Cas9 system, has received widespread attention due to its ability to precisely regulate the cellular functions. However, the precise control of the system in human body still faces challenges. Combined with magnetothermal therapy, its high permeability and low toxicity can be utilized to provide a new cancer treatment method by controlling the thermal effects and synergistically utilizing CRISPR-Cas9 technology. This magnetothermal - gene editing synergistic therapy demonstrates the promising therapeutic potential and provides a new strategy for precision medicine in cancer. Recently, a research paper entitled Magnetothermal activated gene editing strategy for enhanced turbine cell apoptosis, was published in Journal of Nanobiotechnology (Top of Zone 1, if: 1...

[Research Frontier] New Trends in Prime Editing: Point Mutated Cells for Preclinical Gene Therapy

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  Prime editing is an improved gene editing technology based on the CRISPR/Cas system to achieve precise fragment insertion, deletion, and arbitrary substitution of bases at the target site of the genome, with higher accuracy and lower error rate. When it came out, it attracted widespread attention from many scientific researchers, and extensive research was conducted on the treatment of hereditary diseases, gene mutation diseases, and cancers. For example, correcting the mutant genes to treat genetic diseases or editing oncogenes in cancer cell genomes to prevent them from growing and spreading. The following three articles are about the clinical treatment of Prime Editing, bringing you the latest research trends.   Prime editing helps in the treatment of β - thalassemia, and no off-target  effect was detected         β - thalassemia is a hematopoietic system disease caused by a single gene mutation in the hemoglobin (HGB) subunit beta gene (HBB), res...

Development of CRISPR detection | EDITGENE

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CRISPR detection technology is a gene editing technology based on the CRISPR-Cas system for precise editing and detection in the genome. The development of CRISPR detection technology can be divided into the following stages: Initial discovery stage: In the 1980s, scientists discovered the CRISPR-Cas system. This discovery laid the foundation for subsequent gene editing technology. Technology development stage: In the early 21st century, with the development of genomics and molecular biology, scientists began to study how to use the CRISPR-Cas system for gene editing. After a series of research and exploration, the CRISPR-Cas9 system was finally developed, a powerful gene editing tool that can achieve precise editing and detection in the genome. Application stage: As the CRISPR-Cas9 system continues to be improved and optimized, its application scope is becoming more and more extensive. At present, CRISPR detection technology has been widely used in basic research, biotechnology, medi...

Prime editing 101:what is prime editing? | EDITGENE

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Prime editing is an   advanced   gene editing technology proposed by David R. Liu's team in 2019. It can mediate targeted insertions, deletions, and twelve base substitutions without DSBs and donor DNA templates to achieve precise gene editing. Prime editing has developed rapidly in the past 4   years and has shown great potential in various fields.   Prime Editing  mechanism The prime editing system consists of Prime editing guide RNA (pegRNA) and nCas9. PegRNA can be divided into a guide RNA at the 5' end, a Cas9 binding region in the middle, and a PBS (Prime-binding site) at the 3' end; nCas9 is a Cas9 nickase with single-stranded cleavage activity and fused with M-MLV reverse transcriptase. The complex recognizes PAM under the g uidance of pegRNA   and binds to the target DNA strand complementary to the guide RNA. Cas9 enzyme cuts the 3rd and 4th nucleotides of the original spacer sequence upstream of PAM to generate a nick; the broken DNA single strand...